                         SEQUENCE LISTING

<110>  SENTINEXT THERAPEUTICS Sdn Bhd
       
<120>  CHIMERIC ENTEROVIRUS VIRUS-LIKE PARTICLES

<130>  SENTINEXT 7 MY

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  5314
<212>  DNA
<213>  UNKNOWN

<220>
<223>  bac SXT8


<220>
<221>  attB1 recombination site
<222>  (1)..(25)

<220>
<221>  EV-A71 VP4-VP2-VP3(partial)
<222>  (107)..(1660)

<220>
<221>  CV-A16 VP3(partial)
<222>  (1661)..(1801)

<220>
<221>  CV-A16 VP1
<222>  (1802)..(2695)

<220>
<221>  IRES
<222>  (2696)..(3281)

<220>
<221>  EV-A71 3C-3D
<222>  (3282)..(5222)

<220>
<221>  attB2 recombination site
<222>  (5289)..(5314)

<400>  1
acaagtttgt acaaaaaagc aggctagatc ttaatacgac tcactatatt aattaaggat       60

cccaaaaaat tgaaatttta tttttttttt ttggaatata aataatatgg gctcgcaagt      120

cagcactcag aggtcgggct cacacgaaaa cagcaactcg gcaacggaag gctcaactat      180

caactacacc actatcaact actacaagga cagttacgct gccaccgcag gcaagcagtc      240

gctgaaacaa gaccctgata agttcgctaa ccccgtgaaa gacatcttca ctgaaatggc      300

agcgccactc aagagtccgt cggcggaggc ttgcggatac tctgatcgtg tcgctcagtt      360

gaccattggt aactcaacta tcaccactca agaagctgcc aacatcattg tcggctacgg      420

agagtggccc tcttactgtt ccgacagcga tgccaccgca gttgacaagc ctacccgccc      480

tgatgtgtca gtcaacagat tctacacact ggacacgaag ctctgggaaa agtccagcaa      540

aggctggtac tggaaattcc cagatgtgtt gacagagacg ggagtcttcg gtcagaacgc      600

ccaattccac tacctgtaca ggagtggatt ctgcatccac gtgcagtgta acgcttcgaa      660

gttccatcaa ggtgccctgc tcgttgcagt gctgcctgaa tacgtcatcg gaaccgttgc      720

cggtggcacc ggtactgagg acacacaccc tccctacaaa cagactcaac ctggtgccga      780

cggattcgag ctgcagcatc catacgtcct cgatgctgga atccctattt cccagctgac      840

cgtttgcccc caccaatgga ttaacctccg cacaaacaac tgtgctacga tcattgtgcc      900

atacatcaac gcattgccgt tcgactccgc gctgaaccat tgcaacttcg gtttgctggt      960

ggtccctatc agccccctgg actacgatca gggcgcaacc ccagtgatcc cgattacaat     1020

cacgttggcg cctatgtgtt ctgagttcgc tggactgagg caggcagtca cccaaggatt     1080

ccctactgag ttgaagcccg gtactaacca attcctgaca acggacgatg gagtcagcgc     1140

tcctatcctg cccaacttcc accctacacc ctgcattcat atccccggtg aagttcgtaa     1200

cctcttggag ctgtgtcagg tggagaccat cctcgaagtt aacaacgtgc caaccaacgc     1260

tacttccttg atggaaaggc tgagattccc tgtgagcgct caagctggca agggtgagct     1320

gtgcgctgtc ttcagggctg acccaggtcg taacggaccg tggcagagca cactgctggg     1380

acaactctgt ggatactaca cgcagtggtc tggttcattg gaagtgacct tcatgttcac     1440

tggctccttc atggcaacag gaaagatgtt gatcgcgtac acgccaccgg gaggtccatt     1500

gccgaaagac cgcgccacag caatgctggg cacgcacgtc atctgggatt tcggactcca     1560

atcttcagtc accttggtta ttccttggat ctccaacact cactacaggg cgcatgctag     1620

agacggtgtc ttcgattact acaccactgg cctggtgtcc atatggtacc agacgaacta     1680

cgtcgttcct atcggagctc ccacaacggc ctacattgtg gcactggcag cggctcaaga     1740

caacttcacc atgaagctct gcaaagacac agaggatatc gaacagacgg cgaacattca     1800

aggtgacccc atcgctgaca tgattgatca gacagtcaac aaccaagtta accgctccct     1860

gactgctctc caggtgttgc ctactgcagc aaacactgag gcctcttcac accgtctcgg     1920

cacaggagtg gtccccgcat tgcaagcggc tgaaaccgga gcgagttcga acgcttccga     1980

caagaacctg atcgagaccc gctgcgtcct caaccaccat agcacacagg aaacggccat     2040

tggaaacttc ttctctcgtg caggtctggt ttcaatcatt actatgccta ccactggcac     2100

ccagaacact gacggatacg tcaactggga catcgatctc atgggctacg ctcaattgcg     2160

ccgtaagtgt gagctgttca cctacatgcg cttcgatgcc gaattcactt tcgttgtggc     2220

aaaacctaac ggagagctgg tgccccagct gctccaatac atgtacgtcc ctcccggtgc     2280

tcctaagcca acttcccgtg acagcttcgc ctggcagacc gcaactaacc ctagtgtttt     2340

cgtgaaaatg acagatccac cggcccaggt ctcggttcca ttcatgtctc cggcatcagc     2400

gtaccaatgg ttctacgacg gttaccccac cttcggcgag cacctccagg ctaacgactt     2460

ggattacggc caatgcccaa acaacatgat gggaaccttc tctatcagga cagtgggtac     2520

ggaaaagagt ccccattcga ttactctgag ggtctacatg agaatcaaac acgttagagc     2580

ctggattcca aggccgctca gaaaccagcc atacttgttc aagactaacc ctaactacaa     2640

aggcaacgat attaagtgta cctcgactag ccgtgataaa attactactc tgtaagcccc     2700

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgtg     2760

tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa     2820

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg     2880

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca     2940

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc     3000

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt     3060

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtagt caacaagggg     3120

ctgaaggatg cccagaaggt accccattgt atgggaatct gatctggggc ctcggtgcac     3180

atgctttaca tgtgtttagt cgaggttaaa aaagctctag gccccccgaa ccacggggac     3240

gtggttttcc tttgaaaaac acgatgataa gcttgccaca aatgggtcca agtcttgact     3300

ttgctctttc cctgctgaga aggaacatca ggcaagtcca aacagatcag ggccatttta     3360

ccatgttggg tgtcagggat cgcttggctg ttctcccaag gcacgcacag cccggcaaga     3420

ctatttgggt ggagcacaaa cttgtgaacg tcctcgacgc aatcgagctg gtggatgaac     3480

agggcgttaa tttggaactc acactggtga cacttgacac taatgaaaaa tttagagata     3540

tcaccaagtt cattccagag accattagtg gcgctagtga tgcaactttg gtgatcaata     3600

cagaacatat gccatcaatg tttgtcccag tgggcgacgt cgtgcagtat ggattcttga     3660

accttagtgg aaagccaaca cataggacca tgatgtacaa tttccctaca aaagcaggac     3720

aatgtggagg tgtggtcaca tccgtcggta agatcgttgg tattcacatt ggcggcaacg     3780

gccgccaagg tttctgtgct ggtttgaaga ggagttactt cgcaagtgtg cagggtgaga     3840

tccaatgggt gaaacctaac aaagaaactg gtagactgaa catcaatgga ccaactcgca     3900

ctaagctgga gcctagtgtg tttcatgaag tgtttgaagg caataaggaa ccagcagtct     3960

tgacaagtaa agaccctaga ctggaggtcg actttgagca agccctgttt tccaagtatg     4020

tgggcaatgt tttgcatgag cccgatgaat acgtgactca agctgccctc cactatgcga     4080

atcaactcaa acagttggac ataaacacta gcaagatgag catggaggaa gcgtgctatg     4140

gcactgagaa cctggaagca attgatctct gtactagtgc cggatatcca tacagcgccc     4200

ttggcatcaa gaaaagagac attctcgacc ccgtaaccag ggatgtgtct aagatgaaat     4260

tctatatgga caaatacggc ctcgatctgc catactccac ctacgtaaag gatgagcttc     4320

gctctctgga taaaatcaag aaaggaaagt cacgcctgat agaggctagt agcttgaatg     4380

actctgtcta cctcagaatg acttttggcc acctttacga ggtatttcat gctaaccctg     4440

gcactgtgac cggttccgca gtgggttgca acccagacgt gttttggagt aaacttccga     4500

tccttctgcc tggctcactc tttgcctttg actactcagg atatgatgct agcctcagcc     4560

cggtatggtt cagggccctt gaaattgtgt tgcgcgaaat tggctatcca gaagaggccg     4620

tgtcccttat agaaggaatc aaccacactc accacgtgta ccgcaacaaa acatactgtg     4680

tacttggtgg catgccctca ggctgttctg gtacttccat ctttaactca atgatcaaca     4740

acatcatcat tagaaccctc ttgatcaaaa cctttaaggg aatagacctg gatgagttga     4800

acatggtggc ctatggcgac gatgtgttgg ctagttatcc ctttcctata gattgccttg     4860

agttggctaa gactggcaaa gagtatggtt tgactatgac gcctgcagac aaatcaccct     4920

gtttcaatga agtgacgtgg gaaaatgcta ccttccttaa gagaggcttc ttgccagacc     4980

accaatttcc attcttgatt caccctacga tgcccatgag agagatccat gagtccattc     5040

gctggactaa agacgcacgc aatacccaag accacgtgcg ctccctgtgt ctgttggcat     5100

ggcacaacgg taaggatgaa tatgagaaat ttgtgagtgc aattagatca gttccagttg     5160

gaaaagcgtt ggccattcct aattttgaga atttgagaag aaattggctc gaattgtttt     5220

aaatcgattt aattaatagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     5280

ttggaattca cccagctttc ttgtacaaag tggt                                 5314


<210>  2
<211>  5314
<212>  DNA
<213>  UNKNOWN

<220>
<223>  bacSXT7


<220>
<221>  attB1 recombination site
<222>  (1)..(25)

<220>
<221>  CV-A16 VP4-VP2-VP3(partial)
<222>  (107)..(1660)

<220>
<221>  EV-A71 VP3(partial)
<222>  (1661)..(1801)

<220>
<221>  EV-A71 VP1
<222>  (1802)..(2695)

<220>
<221>  IRES
<222>  (2696)..(3281)

<220>
<221>  EV-A71 3C-3D
<222>  (3282)..(5222)

<220>
<221>  attB2 recombination site
<222>  (5289)..(5314)

<400>  2
acaagtttgt acaaaaaagc aggctagatc ttaatacgac tcactatatt aattaaggat       60

cccaaaaaat tgaaatttta tttttttttt ttggaatata aataatatgg gctcacaagt      120

cagcacgcaa cgcagcggaa gccacgagaa cagcaactcg gcatcggaag gctctactat      180

caactacact acaatcaact actacaagga cgcgtacgct gccagtgctg gtaggcagga      240

catgtcgcaa gatcctaaga aattcaccga ccccgtgatg gatgtcatcc atgagatggc      300

tcctcccttg aagtccccaa gcgccgaagc atgcggctac tccgacagag tggcccagct      360

gacaatcgga aacagcacga ttaccactca agaggcagcg aacatcgtga ttgcttacgg      420

cgagtggcca gaatactgtc cggacacaga tgcgacggct gtcgacaagc ctacccgccc      480

tgatgtctcc gttaacagat tcttcaccct ggacactaag tcctgggcca aggatagcaa      540

aggatggtac tggaaattcc ctgacgtgct cactgaagtg ggtgtcttcg gccagaacgc      600

tcaattccac tacttgtacc gttctggctt ctgcgttcat gtgcagtgta acgcctcaaa      660

gttccaccaa ggagcactgc tcgtcgcggt tctgccagag tacgtcctcg gtaccatcgc      720

tggtggcact ggcaacgaaa acagccatcc accgtacgcg acaacgcagc ctggacaagt      780

tggtgctgtg ctgactcacc cctacgtcct cgacgccggt atcccattgt ctcagctgac      840

agtttgccct catcaatgga ttaacctgag gacaaacaac tgtgctacga tcattgtgcc      900

atacatgaac accgtcccgt tcgactctgc cctgaaccac tgcaacttcg gattgctggt      960

catcccagtg gtcccgctcg atttcaacgc cggtgcaact tcagagatcc caattacagt     1020

gacgatcgcc ccgatgtgtg ctgaattcgc tggactgagg caggcagtca agcaaggaat     1080

ccctaccgag ttgaaacccg gtactaacca gttcctgacc actgacgatg gcgtgagcgc     1140

tcctatcctc cccggattcc accccacccc tcccatccat attccaggag aggtccacaa     1200

cctcttggaa atctgcaggg ttgagacaat tttggaagtg aacaacctga agacgaacga     1260

gacaacgcct atgcagagat tgtgcttccc cgtgagtgtc caatcgaaaa caggcgaact     1320

gtgcgctgcc ttccgcgctg acccaggccg tgatggacct tggcagagta ccatcctcgg     1380

tcaattgtgt cgctactaca cccagtggtc tggctcactc gaagtgactt tcatgttcgc     1440

cggatccttc atggcaaccg gcaagatgtt gatcgcctac actccaccgg gaggtaacgt     1500

gcctgctgac cgtatcaccg ctatgctggg tactcacgtt atttgggatt tcggcttgca     1560

atccagcgtg acactggttg tgccatggat ctcaaacact cactacaggg ctcatgctag     1620

ggcaggatac ttcgactact acaccactgg tatcattacc atatggtatc agactaacta     1680

cgttgtgcct atcggtgctc ctaacacagc atacatcatt gcgctcgcag cggctcaaaa     1740

gaacttcacc atgaagctct gcaaagacgc ttcagatatt ttgcagaccg gtactatcca     1800

aggcgaccgc gtggctgatg tcattgagag ttcgatcggt gacagtgtgt cgcgcgccct     1860

cactcatgcg ttgcctgctc ccacaggcca gaacacgcaa gtgtccagcc accgtctgga     1920

tacaggcaag gtccctgctc tgcaggctgc agaaattgga gcttcttcaa acgccagtga     1980

cgagtcgatg atcgaaacac gctgcgtgct gaactcccat agcacggctg agacaacgct     2040

cgactccttc ttcagccgtg ccggattggt cggtgaaatc gatctccctt tgaagggcac     2100

cactaacccc aacggctacg ctaactggga cattgacatc accggctacg cccagatgcg     2160

ccgtaaggtt gaactcttca cctacatgag gttcgacgct gagttcactt tcgtggcctg     2220

tacacccacg ggcgaagtcg ttccacagtt gctgcaatac atgttcgtgc ctcctggtgc     2280

tcctaagcct gactcccgcg agtccctggc atggcagacc gcgactaacc cctctgtctt     2340

cgttaaactc tcagatccac cggcccaggt gtctgtccca ttcatgagtc cggcctcggc     2400

ataccaatgg ttctacgacg gataccccac cttcggagag cacaagcaag aaaaggacct     2460

ggagtacgga gcttgcccaa acaacatgat gggtactttc agcgttagga cagtgggcac     2520

gtctaagtca aaatacccat tggttgttag gatctacatg agaatgaagc acgtgagagc     2580

ttggattcct cgccccatgc gtaaccagaa ctacctgttc aaagctaacc ctaactacgc     2640

aggcaacagt atcaagccca cgggagcatc aagaacggca atcaccactt tgtaagcccc     2700

tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgtg     2760

tttgtctata tgtgattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa     2820

cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg     2880

caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca     2940

acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc     3000

ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt     3060

gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtagt caacaagggg     3120

ctgaaggatg cccagaaggt accccattgt atgggaatct gatctggggc ctcggtgcac     3180

atgctttaca tgtgtttagt cgaggttaaa aaagctctag gccccccgaa ccacggggac     3240

gtggttttcc tttgaaaaac acgatgataa gcttgccaca aatgggtcca agtcttgact     3300

ttgctctttc cctgctgaga aggaacatca ggcaagtcca aacagatcag ggccatttta     3360

ccatgttggg tgtcagggat cgcttggctg ttctcccaag gcacgcacag cccggcaaga     3420

ctatttgggt ggagcacaaa cttgtgaacg tcctcgacgc aatcgagctg gtggatgaac     3480

agggcgttaa tttggaactc acactggtga cacttgacac taatgaaaaa tttagagata     3540

tcaccaagtt cattccagag accattagtg gcgctagtga tgcaactttg gtgatcaata     3600

cagaacatat gccatcaatg tttgtcccag tgggcgacgt cgtgcagtat ggattcttga     3660

accttagtgg aaagccaaca cataggacca tgatgtacaa tttccctaca aaagcaggac     3720

aatgtggagg tgtggtcaca tccgtcggta agatcgttgg tattcacatt ggcggcaacg     3780

gccgccaagg tttctgtgct ggtttgaaga ggagttactt cgcaagtgtg cagggtgaga     3840

tccaatgggt gaaacctaac aaagaaactg gtagactgaa catcaatgga ccaactcgca     3900

ctaagctgga gcctagtgtg tttcatgaag tgtttgaagg caataaggaa ccagcagtct     3960

tgacaagtaa agaccctaga ctggaggtcg actttgagca agccctgttt tccaagtatg     4020

tgggcaatgt tttgcatgag cccgatgaat acgtgactca agctgccctc cactatgcga     4080

atcaactcaa acagttggac ataaacacta gcaagatgag catggaggaa gcgtgctatg     4140

gcactgagaa cctggaagca attgatctct gtactagtgc cggatatcca tacagcgccc     4200

ttggcatcaa gaaaagagac attctcgacc ccgtaaccag ggatgtgtct aagatgaaat     4260

tctatatgga caaatacggc ctcgatctgc catactccac ctacgtaaag gatgagcttc     4320

gctctctgga taaaatcaag aaaggaaagt cacgcctgat agaggctagt agcttgaatg     4380

actctgtcta cctcagaatg acttttggcc acctttacga ggtatttcat gctaaccctg     4440

gcactgtgac cggttccgca gtgggttgca acccagacgt gttttggagt aaacttccga     4500

tccttctgcc tggctcactc tttgcctttg actactcagg atatgatgct agcctcagcc     4560

cggtatggtt cagggccctt gaaattgtgt tgcgcgaaat tggctatcca gaagaggccg     4620

tgtcccttat agaaggaatc aaccacactc accacgtgta ccgcaacaaa acatactgtg     4680

tacttggtgg catgccctca ggctgttctg gtacttccat ctttaactca atgatcaaca     4740

acatcatcat tagaaccctc ttgatcaaaa cctttaaggg aatagacctg gatgagttga     4800

acatggtggc ctatggcgac gatgtgttgg ctagttatcc ctttcctata gattgccttg     4860

agttggctaa gactggcaaa gagtatggtt tgactatgac gcctgcagac aaatcaccct     4920

gtttcaatga agtgacgtgg gaaaatgcta ccttccttaa gagaggcttc ttgccagacc     4980

accaatttcc attcttgatt caccctacga tgcccatgag agagatccat gagtccattc     5040

gctggactaa agacgcacgc aatacccaag accacgtgcg ctccctgtgt ctgttggcat     5100

ggcacaacgg taaggatgaa tatgagaaat ttgtgagtgc aattagatca gttccagttg     5160

gaaaagcgtt ggccattcct aattttgaga atttgagaag aaattggctc gaattgtttt     5220

aaatcgattt aattaatagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     5280

ttggaattca cccagctttc ttgtacaaag tggt                                 5314


<210>  3
<211>  5371
<212>  DNA
<213>  UNKNOWN

<220>
<223>  bacSXT11


<220>
<221>  attB1recombination site
<222>  (1)..(25)

<220>
<221>  PV1 P1
<222>  (107)..(2749)

<220>
<221>  IRES
<222>  (2753)..(3338)

<220>
<221>  EV-A71 3C-3D
<222>  (3339)..(5279)

<220>
<221>  attB2 recombination site
<222>  (5346)..(5371)

<400>  3
acaagtttgt acaaaaaagc aggctagatc ttaatacgac tcactatatt aattaaggat       60

cccaaaaaat tgaaatttta tttttttttt ttggaatata aataatatgg gagcacaggt      120

ctcatcacag aaggtcggag cacacgagaa cagtaacagg gcctacggag gaagtacaat      180

caactacacc accatcaact actatcgcga ctctgcctca aatgccgcta gcaagcagga      240

ctttagtcag gatccaagca aattcactga gcccatcaag gacgtgctca ttaaaacagc      300

accaatgctg aactccccca atatcgaagc ctgcggctac tctgataggg tcctgcagct      360

caccctggga aactcaacta ttaccactca ggaggcagcc aatagtgtgg tcgcatacgg      420

tcgctggcct gagtatctga gggactccga agccaaccca gtggaccagc ccactgaacc      480

tgatgtcgct gcatgtagat tctacacact ggagaccgtg tcatggacaa aagaaagtaa      540

aggctggtgg tggaagctcc cagacgctct gcgcgatatg ggtctcttcg gccagaacat      600

gtactatcac tacctgggaa ggtcaggtta caccgtgcac gtccagtgca atgccagtaa      660

atttcatcag ggcgcactcg gagtgttcgc cgtccctgag atgtgcctgg ccggagactc      720

taacacaacc cccactcata catcatacca gaacgcaaat cctggcgaaa agggcggaac      780

ctttactgga acattcaccc cagacaacaa tcagacaagc cctgctcgca ggttttgccc      840

agtggattac ctgttcggaa acggtaccct gctcggaaat gctttcgtct ttcctcacca      900

gatcattaac ctgaggacaa acaattgtgc caccctcgtg ctgccatacg tcaatagtct      960

gagcatcgac tccatggtga agcataacaa ttggggaatt gctatcattc cactggcacc     1020

cctcaacttc gccaatgaga gcagccctga aatcccaatt actctgacaa tcgccccaat     1080

gtgctgtgag ttcaacggtc tgagaaatat taccctgccc cgtctccagg gtctgcctgt     1140

gatgaacaca ccaggcagca atcagtacct caccgccgat aactttcagt ccccatgcgc     1200

tctgcccgag ttcgacgtga cccctcccat cgatattccc ggcgaagtca agaacatgat     1260

ggagctggcc gaaatcgaca ctatgattcc tttcgatctg agtgctacta agaaaaatac     1320

aatggagatg taccgggtgc agctgtcaga caaaccccac accgacgatc ctatcctctg     1380

cctgtccctc tctcctgcta ccgatccaag gctgagccac actatgctcg gtgaaattct     1440

gaactactat acacattggg ccggcagcct caagttcacc tttctgttct gtggatccat     1500

gatggctacc ggtaaactgc tcgtgtctta cgccccaccc ggtgctgacc ctccaaagaa     1560

aagaaaggag gccatgctcg gaacccacgt catctgggat attggtctgc agtcttcatg     1620

cactatggtg gtcccctgga tctccaacac tacataccgt cagactattg acgatagttt     1680

tacagagggt ggctacatca gcgtgttcta tcagactcgg attgtggtcc ccctctctac     1740

acctcgcgaa atggacatcc tgggattcgt gagcgcctgt aacgacttct ccgtcaggct     1800

gctcagagat accactcaca tcgagcagaa ggcaattgcc cagggcctcg gacagatgct     1860

ggagagctcc atcgacaaca ccgtgaggga aactgtcggc gcttcaacca gtagagatgc     1920

actgccaaat actgaatcct ctggacccgc acattctaag gagatccctg cactgaccgc     1980

cgtggaaact ggcgccacaa accccctggt gcctagcgac accgtccaga ctagacacgt     2040

gatccagcat cggagccgct ccgagtcaag tgtggaatct ttctttgctc gtggagcatg     2100

cgtcacaatc atgaccgtgg acaactctgc ttcaacaacc tctaaggata aactgttctc     2160

agtgtggaag atcacataca aagacaccgt ccagctcagg cgtaagctgg agttctttac     2220

ctattcccgc tttgatatgg aatttacttt cgtgatcaca gctaacttca ccgagactaa     2280

caatggtcac gcactgaatc aggtctacca gattatgtat gtgccccctg gcgcccctgt     2340

cccagaaaag tgggacgatt acacttggca gacaagctcc aacccctcta tcttctacac     2400

ctatggcact gctcccgcaa gaatctcagt gccttatgtc ggaatttcca atgcttactc     2460

tcacttttat gacggtttca gcaaggtgcc tctgaaagac cagagtgccg agctgggtga     2520

ttccctgtac ggcgccgctt ccctcaacga cttcggaatc ctggccgtgc gtgtggtcaa     2580

cgatcacaat ccaacaaagg tgacctccaa aatccgtgtc tatctgaagc caaaacatat     2640

tcgggtgtgg tgtccacgtc caccccgggc agtcgcttac tatggtcccg gcgtggacta     2700

taaggatggt actctcgcac ctctgagcac taaggatctg actacctatt aagcccctct     2760

ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgtgttt     2820

gtctatatgt gattttccac catattgccg tcttttggca atgtgagggc ccggaaacct     2880

ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa     2940

ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg     3000

tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc     3060

caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg     3120

agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtagtcaa caaggggctg     3180

aaggatgccc agaaggtacc ccattgtatg ggaatctgat ctggggcctc ggtgcacatg     3240

ctttacatgt gtttagtcga ggttaaaaaa gctctaggcc ccccgaacca cggggacgtg     3300

gttttccttt gaaaaacacg atgataagct tgccacaaat gggtccaagt cttgactttg     3360

ctctttccct gctgagaagg aacatcaggc aagtccaaac agatcagggc cattttacca     3420

tgttgggtgt cagggatcgc ttggctgttc tcccaaggca cgcacagccc ggcaagacta     3480

tttgggtgga gcacaaactt gtgaacgtcc tcgacgcaat cgagctggtg gatgaacagg     3540

gcgttaattt ggaactcaca ctggtgacac ttgacactaa tgaaaaattt agagatatca     3600

ccaagttcat tccagagacc attagtggcg ctagtgatgc aactttggtg atcaatacag     3660

aacatatgcc atcaatgttt gtcccagtgg gcgacgtcgt gcagtatgga ttcttgaacc     3720

ttagtggaaa gccaacacat aggaccatga tgtacaattt ccctacaaaa gcaggacaat     3780

gtggaggtgt ggtcacatcc gtcggtaaga tcgttggtat tcacattggc ggcaacggcc     3840

gccaaggttt ctgtgctggt ttgaagagga gttacttcgc aagtgtgcag ggtgagatcc     3900

aatgggtgaa acctaacaaa gaaactggta gactgaacat caatggacca actcgcacta     3960

agctggagcc tagtgtgttt catgaagtgt ttgaaggcaa taaggaacca gcagtcttga     4020

caagtaaaga ccctagactg gaggtcgact ttgagcaagc cctgttttcc aagtatgtgg     4080

gcaatgtttt gcatgagccc gatgaatacg tgactcaagc tgccctccac tatgcgaatc     4140

aactcaaaca gttggacata aacactagca agatgagcat ggaggaagcg tgctatggca     4200

ctgagaacct ggaagcaatt gatctctgta ctagtgccgg atatccatac agcgcccttg     4260

gcatcaagaa aagagacatt ctcgaccccg taaccaggga tgtgtctaag atgaaattct     4320

atatggacaa atacggcctc gatctgccat actccaccta cgtaaaggat gagcttcgct     4380

ctctggataa aatcaagaaa ggaaagtcac gcctgataga ggctagtagc ttgaatgact     4440

ctgtctacct cagaatgact tttggccacc tttacgaggt atttcatgct aaccctggca     4500

ctgtgaccgg ttccgcagtg ggttgcaacc cagacgtgtt ttggagtaaa cttccgatcc     4560

ttctgcctgg ctcactcttt gcctttgact actcaggata tgatgctagc ctcagcccgg     4620

tatggttcag ggcccttgaa attgtgttgc gcgaaattgg ctatccagaa gaggccgtgt     4680

cccttataga aggaatcaac cacactcacc acgtgtaccg caacaaaaca tactgtgtac     4740

ttggtggcat gccctcaggc tgttctggta cttccatctt taactcaatg atcaacaaca     4800

tcatcattag aaccctcttg atcaaaacct ttaagggaat agacctggat gagttgaaca     4860

tggtggccta tggcgacgat gtgttggcta gttatccctt tcctatagat tgccttgagt     4920

tggctaagac tggcaaagag tatggtttga ctatgacgcc tgcagacaaa tcaccctgtt     4980

tcaatgaagt gacgtgggaa aatgctacct tccttaagag aggcttcttg ccagaccacc     5040

aatttccatt cttgattcac cctacgatgc ccatgagaga gatccatgag tccattcgct     5100

ggactaaaga cgcacgcaat acccaagacc acgtgcgctc cctgtgtctg ttggcatggc     5160

acaacggtaa ggatgaatat gagaaatttg tgagtgcaat tagatcagtt ccagttggaa     5220

aagcgttggc cattcctaat tttgagaatt tgagaagaaa ttggctcgaa ttgttttaaa     5280

tcgatttaat taatagcata accccttggg gcctctaaac gggtcttgag gggttttttg     5340

gaattcaccc agctttcttg tacaaagtgg t                                    5371


<210>  4
<211>  5335
<212>  DNA
<213>  UNKNOWN

<220>
<223>  bacSXT18


<220>
<221>  attB1 recombination site
<222>  (1)..(25)

<220>
<221>  EV-A71 VP4-VP2-VP3-VP1(partial)
<222>  (107)..(1832)

<220>
<221>  PV1 VP1(partial)
<222>  (1833)..(2713)
<223>  A1832T and T1833C introduced a SacI restriction site.  Double 
       mutation leads to a Met to Ser amino acid substitution in PV1 
       VP1.

<220>
<221>  IRES
<222>  (2717)..(3302)

<220>
<221>  EV-A71 3C-3D
<222>  (3303)..(5243)

<220>
<221>  attB2 recombination site
<222>  (5310)..(5335)

<400>  4
acaagtttgt acaaaaaagc aggctagatc ttaatacgac tcactatatt aattaaggat       60

cccaaaaaat tgaaatttta tttttttttt ttggaatata aataatatgg gctcacaggt      120

gtccactcaa cgctccggct cccatgaaaa ctctaattca gctacagaag gctccaccat      180

taattacacc actattaact attacaaaga ttcctatgct gcgacagccg gcaaacagag      240

ccttaaacaa gaccctgata agtttgctaa ccctgtcaag gacattttca ctgaaatggc      300

tgcgcctctt aagtctccat ccgctgaagc ttgtggttac agtgatcgcg tggcacaact      360

caccattgga aactccacca ttactacaca ggaagcagca aacatcatag tcggttatgg      420

tgaatggccc tcatactgct ccgatgacga cgctacagcg gtggataagc caacgcgccc      480

agatgtttcc gtgaataggt tttatacgtt ggacactaaa ctgtgggaaa agtcatccaa      540

gggctggtat tggaagtttc ctgatgtatt gactgagacc ggagtctttg gccaaaacgc      600

acaatttcac tatttgtata gatcaggctt ttgcatccat gtgcaatgta acgctagcaa      660

gttccaccaa ggagcgctgt tggtcgctat acttccagag tatgtcatag gcacggtggc      720

aggcggtaca ggaacagagg acagtcaccc tccttacaaa caaacacaac caggtgccga      780

cggttttgaa ttgcagcacc cgtatgtgct cgatgctgga atccctatat cacaattgac      840

agtatgcccc catcaatgga ttaacctgcg caccaataac tgtgccacaa taatagtgcc      900

atatatgaat acactgcctt ttgactccgc cctgaaccat tgcaattttg gcctgttggt      960

agtacccatt agcccattgg attttgacca aggcgcaact ccggttatcc ctattacaat     1020

cactcttgct ccaatgtgct ctgagtttgc tggtctcagg caggcagtca ctcaaggctt     1080

tcccactgag ccaaaaccag gcacgaacca attcttgacc accgatgatg gcgtctcagc     1140

acccattctg ccaaatttcc atcccactcc atgtattcac atacccggtg aagtcagaaa     1200

cctgctcgag ttgtgtcaag tggagactat tcttgaggtt aacaacgtac ccaccaatgc     1260

tactagtttg atggaaagac ttcgcttccc ggtgtccgcg caagcgggca aaggtgaatt     1320

gtgtgctgtg tttagggctg atcctggaag agacggtcca tggcagtcaa cgatgctggg     1380

ccagttgtgt ggatactaca ctcagtggtc aggctcactg gaggtcactt tcatgttcac     1440

cggctctttc atggccacgg gtaagatgct catagcttat acacctcctg gcggtccctt     1500

gcccaaagat cgcgctacag caatgctggg cacacatgtt atttgggatt ttggccttca     1560

atcatctgtc acccttgtga taccatggat cagtaatacc cactacagag cgcatgccag     1620

ggatggagtg ttcgactact acaccacagg actggtcagt atctggtacc aaacaaatta     1680

cgtagttcca attggcgcac ccaacacagc ttacataata gcactggcgg cggcccagaa     1740

gaatttcacc atgaaactgt gcaaagacac tagtcacata ttgcagacag cctctattca     1800

gggagatagg gtggcagatg tgatcgagag ctccatcgac aacaccgtga gggaaactgt     1860

cggcgcttca accagtagag atgcactgcc aaatactgaa tcctctggac ccgcacattc     1920

taaggagatc cctgcactga ccgccgtgga aactggcgcc acaaaccccc tggtgcctag     1980

cgacaccgtc cagactagac acgtgatcca gcatcggagc cgctccgagt caagtgtgga     2040

atctttcttt gctcgtggag catgcgtcac aatcatgacc gtggacaact ctgcttcaac     2100

aacctctaag gataaactgt tctcagtgtg gaagatcaca tacaaagaca ccgtccagct     2160

caggcgtaag ctggagttct ttacctattc ccgctttgat atggaattta ctttcgtgat     2220

cacagctaac ttcaccgaga ctaacaatgg tcacgcactg aatcaggtct accagattat     2280

gtatgtgccc cctggcgccc ctgtcccaga aaagtgggac gattacactt ggcagacaag     2340

ctccaacccc tctatcttct acacctatgg cactgctccc gcaagaatct cagtgcctta     2400

tgtcggaatt tccaatgctt actctcactt ttatgacggt ttcagcaagg tgcctctgaa     2460

agaccagagt gccgagctgg gtgattccct gtacggcgcc gcttccctca acgacttcgg     2520

aatcctggcc gtgcgtgtgg tcaacgatca caatccaaca aaggtgacct ccaaaatccg     2580

tgtctatctg aagccaaaac atattcgggt gtggtgtcca cgtccacccc gggcagtcgc     2640

ttactatggt cccggcgtgg actataagga tggtactctc gcacctctga gcactaagga     2700

tctgactacc tattaagccc ctctccctcc ccccccccta acgttactgg ccgaagccgc     2760

ttggaataag gccggtgtgt gtttgtctat atgtgatttt ccaccatatt gccgtctttt     2820

ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt     2880

tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg     2940

gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca     3000

cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg     3060

gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc     3120

tcaagcgtag tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggaatc     3180

tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaagctcta     3240

ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata agcttgccac     3300

aaatgggtcc aagtcttgac tttgctcttt ccctgctgag aaggaacatc aggcaagtcc     3360

aaacagatca gggccatttt accatgttgg gtgtcaggga tcgcttggct gttctcccaa     3420

ggcacgcaca gcccggcaag actatttggg tggagcacaa acttgtgaac gtcctcgacg     3480

caatcgagct ggtggatgaa cagggcgtta atttggaact cacactggtg acacttgaca     3540

ctaatgaaaa atttagagat atcaccaagt tcattccaga gaccattagt ggcgctagtg     3600

atgcaacttt ggtgatcaat acagaacata tgccatcaat gtttgtccca gtgggcgacg     3660

tcgtgcagta tggattcttg aaccttagtg gaaagccaac acataggacc atgatgtaca     3720

atttccctac aaaagcagga caatgtggag gtgtggtcac atccgtcggt aagatcgttg     3780

gtattcacat tggcggcaac ggccgccaag gtttctgtgc tggtttgaag aggagttact     3840

tcgcaagtgt gcagggtgag atccaatggg tgaaacctaa caaagaaact ggtagactga     3900

acatcaatgg accaactcgc actaagctgg agcctagtgt gtttcatgaa gtgtttgaag     3960

gcaataagga accagcagtc ttgacaagta aagaccctag actggaggtc gactttgagc     4020

aagccctgtt ttccaagtat gtgggcaatg ttttgcatga gcccgatgaa tacgtgactc     4080

aagctgccct ccactatgcg aatcaactca aacagttgga cataaacact agcaagatga     4140

gcatggagga agcgtgctat ggcactgaga acctggaagc aattgatctc tgtactagtg     4200

ccggatatcc atacagcgcc cttggcatca agaaaagaga cattctcgac cccgtaacca     4260

gggatgtgtc taagatgaaa ttctatatgg acaaatacgg cctcgatctg ccatactcca     4320

cctacgtaaa ggatgagctt cgctctctgg ataaaatcaa gaaaggaaag tcacgcctga     4380

tagaggctag tagcttgaat gactctgtct acctcagaat gacttttggc cacctttacg     4440

aggtatttca tgctaaccct ggcactgtga ccggttccgc agtgggttgc aacccagacg     4500

tgttttggag taaacttccg atccttctgc ctggctcact ctttgccttt gactactcag     4560

gatatgatgc tagcctcagc ccggtatggt tcagggccct tgaaattgtg ttgcgcgaaa     4620

ttggctatcc agaagaggcc gtgtccctta tagaaggaat caaccacact caccacgtgt     4680

accgcaacaa aacatactgt gtacttggtg gcatgccctc aggctgttct ggtacttcca     4740

tctttaactc aatgatcaac aacatcatca ttagaaccct cttgatcaaa acctttaagg     4800

gaatagacct ggatgagttg aacatggtgg cctatggcga cgatgtgttg gctagttatc     4860

cctttcctat agattgcctt gagttggcta agactggcaa agagtatggt ttgactatga     4920

cgcctgcaga caaatcaccc tgtttcaatg aagtgacgtg ggaaaatgct accttcctta     4980

agagaggctt cttgccagac caccaatttc cattcttgat tcaccctacg atgcccatga     5040

gagagatcca tgagtccatt cgctggacta aagacgcacg caatacccaa gaccacgtgc     5100

gctccctgtg tctgttggca tggcacaacg gtaaggatga atatgagaaa tttgtgagtg     5160

caattagatc agttccagtt ggaaaagcgt tggccattcc taattttgag aatttgagaa     5220

gaaattggct cgaattgttt taaatcgatt taattaatag cataacccct tggggcctct     5280

aaacgggtct tgaggggttt tttggaattc acccagcttt cttgtacaaa gtggt          5335


